Thank you for the helpful reply! I was trying to build my application with PySide6-deploy, which is putting all files into the MacOS subdirectory. After researching I ended up using Py2App, which follows the required folder structure. Providing only the top level MyApp.app/ directory path did not work, after getting a myriad of complaints of the notarization. Do I have to sign now every file individually? And is there a generally preferred tool for packaing Python based apps?